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. Author manuscript; available in PMC: 2021 Nov 22.
Published in final edited form as: Cell Rep. 2021 Oct 5;37(1):109769. doi: 10.1016/j.celrep.2021.109769

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
rabbit α-dmXNP15879 antibody Emelyanov et al., 2010 N/A
rabbit α-dmHIRA antibody Konev et al., 2007 N/A
rabbit α-dmASF1 antibody Jessica Tyler, Weill Cornell Medicine N/A
mouse α-tubulin antibody Sigma Aldrich RRID: AB_477579
HRP-coupled secondary antibody mouse Sigma Aldrich RRID: AB_259755
HRP-coupled secondary antibody rabbit Sigma Aldrich RRID: AB_258103
Chemicals, peptides, and recombinant proteins
NaCl Roth Cat#39572
HEPES-KOH Roth Cat#9105.3
EDTA Sigma Aldrich Cat#E6760
NP-40 Sigma Aldrich Cat#I3021
UltraPure Sucrose Invitrogen Cat#15503022
Propidium iodide Sigma Aldrich Cat#P4170
MgCl2 Roth Cat#2189,1
H2SO4 Merck Cat#30743
Acetone Roth Cat#9372.2
Tris base Sigma Aldrich Cat#GE17-1321-01
Trichloroacetic acid (TCA) Merck Cat#8071000
Ammonium bicarbonate (ABC) Merck Cat#101131
Acetonitrile (ACN) Bartlet Cat#CL0001752500
Dithiothreitol (DTT) Roth Cat#6908.3
Iodoactamide (IAA) Sigma Aldrich Cat#I-6125
Pepsin Promega Cat#V1959
SDS Roth Cat#2326.5
Glycerol Roth Cat#3783.1
2-mercapto-ethanol Roth Cat#4227.3
TRI Reagent Sigma Aldrich Cat#T-9424
DNase I New England Biolabs Cat#M0303
Igepal CA-630 Sigma Aldrich Cat#I8896
cOmplete, EDTA-free protease inhibitor coctail Roche Cat#COEDTAF-RO
Q5 High-Fidelity DNA Polymerase New England Biolabs Cat#E0555
Tegosept Sigma Aldrich Cat#54680
Rapamycin from Streptomyces hygroscopicus Sigma Aldrich Cat#R0395
Triton X-100 Acros Organics Cat#215682500
E133 brilliant blue food coloring Sigma Aldrich Cat#80717
Porcine trehalase Sigma Aldrich Cat#T8778
Amyloglycosidase Sigma Aldrich Cat#A7095
Critical commercial assays
Luna® Universal qPCR Master Mix New England Biolabs Cat#M3003
LunaScript RT SuperMix Kit New England Biolabs Cat#E3010
GE Healthcare Amersham ECL Prime Western-Blot Detection GE Healthcare Cat#28980926
Illumina Tagment DNA Enzyme and Buffer Illumina Cat#20034197
Monarch Genomic DNA Purification Kit New England Biolabs Cat#T3010
Monarch PCR & DNA Cleanup Kit New England Biolabs Cat#T1030
Glucose (HK) assay kit Sigma Aldrich Cat#GAHK20
Serum Triglyceride Detection Kit Sigma Aldrich Cat#TR0100
Deposited data
ATAC-seq raw data Sequence Read Archive SRA SRA: PRJNA730408
RNA-seq data Gene Expression Omnibus repository GEO: GSE146392
Experimental models: organisms/strains
Drosophila melanogaster: Chd1 WT/WT: w1118; Chd11, P{Chd1+} / Df(2L)Exel7014, P{Chd1+} Morettini et al., 2011 N/A
Drosophila melanogaster: Chd1−/−: w1118; Chd11 / Df(2L)Exel7014 Konev et al., 2007 N/A
Drosophila melanogaster: UAS-Chd1: w1118; Chd11, P{w+,UASt-Chd1} / Df(2L)Exel7014 Konev et al., 2007 N/A
Drosophila melanogaster: elav-Gal4: w1118; Chd11 / Df(2L)Exel7014; P{w+, elav-Gal4}/+ this study N/A
Drosophila melanogaster: Chd1elav: w1118; Chd11, P{w+,UASt-Chd1} / Df(2L)Exel7014; P{w+, elav-Gal4}/+ this study N/A
Drosophila melanogaster: H3.3KO: w1118, delH3.3B(w+), hsp-Flp; Df/(2L)H3.3A/SM6B Hödl and Basler, 2009 N/A
Drosophila melanogaster: Ssm185b: w1118, ssm 185b/FM7c Loppin et al., 2000 N/A
Drosophila melanogaster: UAS-2xsNPF: w1118; UAS-sNPF; UAS-sNPF Lee et al., 2004 N/A
Drosophila melanogaster: Chd1sNPF: w1118; Chd1, UAS-sNPF/Df(2L)Exel7014; P{w+, elav-Gal4}/UAS-sNPF this study N/A
Drosophila melanogaster: Oregon R: w+O this study N/A
Oligonucleotides
Ad1_noMX Buenrostro et al., 2013 N/A
Ad2.1-Ad2.3 Buenrostro et al., 2013 N/A
Ad2.7-Ad2.12 Buenrostro et al., 2013 N/A
Software and Algorithms
Skyline Spectral Library MacCoss Lab Software v20.1.0.155
Fusion Software PeqLab v15.17
R https://www.r-project.org v3.6.1
deepTools suite Ramírez et al., 2014 v3.5.1
GraphPad Prism GraphPad v8.2.1