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. 2021 Nov 8;12:753681. doi: 10.3389/fimmu.2021.753681

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Copyright © 2021 Du, Xiang, Xu, Cui, Pang, Xu, Mai and Ai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of SHP on LPS-induced inflammation of large yellow croakers. (A-C) Fluorescence analysis, immunoblots and qRT-PCR assays for SHP in macrophages infected by croaker SHP adenovirus. (D) Expression of tnfα, il-1β, il-6 and cox2 in macrophages after LPS stimulation alone or with SHP overexpression. (E) Expression of il-1β and il-6 in macrophages after LPS stimulation at different time points. Data are presented as means ± SEMs, n = 3 replicate experiments. Significance was evaluated by one-factor ANOVA followed by Duncan’s multiple range test. Labeled means without a common letter differ, P < 0.05. ^∗Different from control (adv-GFP), P < 0.05 (Student’s t-test). cox2, cyclooxygenase 2; LPS, lipopolysaccharide; il-1β, interleukin 1 beta; il6, interleukin 6; SHP, small heterodimer partner; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; tnfa, tumor necrosis factor alpha.