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. 2021 Nov 8;12:753681. doi: 10.3389/fimmu.2021.753681

Figure 8.

Figure 8

LPS induces the expression of SHP through the AMPK signaling pathway. (A) Immunoblots for phosphorylated AMPK in macrophages after LPS stimulation. (B) Immunoblots for phosphorylated AMPK and SHP in macrophages after LPS stimulation alone or with AMPK inhibitor (CC). (C) Expression of shp in macrophages after LPS stimulation or AMPK inhibitor treatment. (D) Effects of AMPK activator (AICAR) on protein levels of phosphorylated AMPK, phosphorylated ACC and SHP in macrophages. (E) Gene expression of shp in macrophages after treatment with AICAR. Data are presented as means ± SEMs, n = 3 replicate experiments. Significance was evaluated by one-factor ANOVA followed by Duncan’s multiple range test. Labeled means without a common letter differ, P < 0.05. ∗Different from control, P < 0.05 (Student’s t-test). ACC, Acetyl-CoA carboxylase; AMPK, Adenosine 5’-monophosphate (AMP)-activated protein kinase; AICAR, 5-aminoimidazole-4-carboxamide ribonucleotide; CC, Compound C; LPS, lipopolysaccharide; SHP, small heterodimer partner; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.