Fig. 1.

Experimental timeline. A The animals were injected with AAV-Htt171-82Q or AAV-GFP into the right striatum and assigned to the HD group (n = 20), sham group (n = 20), and control group (n = 5). Behavioral tests were performed every 2 weeks. The hESCs were transplanted 7 weeks after infection and an MRI scan was conducted for baseline information. SPION cell tracking was managed once per 2 weeks after that and after the third time scanning, the rats were transcardially perfused for histological examinations. B Location of AAV-Htt171-82Q or AAV-GFP injection into the right striatum with stereotaxic coordination. C Location of hESCs transplantation into the right and left striatum with stereotaxic coordination