Fig. 5.

Cytotoxicity and oral mucosa irritation tests of the mineralizing film. a–d CCK-8 assay of L929 and human gingival fibroblasts. e–l Histological sections of oral mucosa from golden hamster cheek pouches. The oral mucosa was treated either with polar (0.9% NaCl) and nonpolar (cottonseed oil) liquid in the control group (e–h), or with the polar and nonpolar extracts of mineralizing film in the experimental group (i–l). f, h, j, and l are magnified images of e, g, i, and k. The stratified squamous epithelium and lamina propria were in normal arrangement. No cell proliferation, no edema, no inflammatory cells and no cell necrosis were detected