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. 2021 Nov 8;12:770051. doi: 10.3389/fphys.2021.770051

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Copyright © 2021 Yang, Li, Chen, Li, Huang and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Classification of single WT cardiomyocytes treated with isoprenaline or vehicle using Ca²⁺ spark characteristics. Statistics and distributions of amplitude (A) and rise time (B) of Ca²⁺ sparks in WT cardiomyocytes treated with 0.1-μmol/L isoprenaline (ISO) or vehicle (VEH). These parameters show obviously different distributions between the two groups. Statistics of t50 (C), FDHM (D), and FWHM (E) display no difference. n = 557 or 675 events from ≥20 cardiomyocytes per group. (F) Statistics of event and cell classification accuracy in the independent test dataset comprising 46 “VEH” and 20 “ISO” treated WT cardiomyocytes from at least five mice.