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. 2021 Nov 21;15:68. doi: 10.1186/s40246-021-00368-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Expression of known marker genes in clusters of 3 brain regions, ACC (anterior cingulate cortex), MTG (middle temporal gyrus) and VISp (primary visual cortex). A Boxplot representing the average expression of cluster genes related to specific cell types (known marker genes); box plots showing median, interquartile range (IQR) with whiskers adding IQR to the 1st and 3rd quartile. Y-axis represents normalised gene expression, x-axis represents known marker genes of brain cell types, oligodendrocytes, astrocytes, OPC, microglia and neurons. B Donut plot displaying the composition of top 20 DE genes in comparison to known marker gene list (yellow-astrocytes, orange-microglia, pink-oligodendrocytes, grey-others). C Feature plot to visualise the expression of known marker gene related to specific cell types on tSNE plot. Expression of astrocytes (ADGRV1), oligodendrocytes (MOG) and microglia (P2RY12/ CX3CR1) across different clusters are also shown