Figure 6. Sox6 progenitor fate map.

(A) Schematic of PRISM alleles.

(B) Sox6-Cre, Nestin-LSL-Flpo, RC-LacZ (from here on Sox6 PRISM) at E11.5. βGal⁺ progenitors (Sox6⁺) are located medially in the Lmx1a⁺ floor plate.

(C) Sox6 PRISM adult brains show TH⁺,βGal⁺ neurons in the SNc and VTA (n = 8).

(C’) High magnification of SNc TH⁺,βGal⁺ cells (arrowheads) and TH⁺,βGal⁻ cells (triangles).

(D) Average TH⁺,βGal⁺/TH⁺ neurons in Sox6 PRISM adult brains, also corrected by PRISM efficiency; p = 0.1.

(E) High magnification of Sox6 PRISM SNc neurons co-expressing TH, ALDH1A1, and βGal.

(F) Sox6 PRISM SNc cells co-expressing dopamine transporter (DAT), CALBINDIN-D28k (CB), and βGal. Most CB⁺,DAT⁺ cells are βGal⁻ (triangles). For reference, arrowhead shows a βGal⁺,DAT⁺,CB⁻ cell.

(G) Percentage of βGal⁺ cells that express ALDH1A1 or CB in the SNc and VTA of Sox6 PRISM adult brains (p values: SNc p = 0.008, VTAp = 0.5).

Scale bars: (C) 200 μm; all other scale bars, 50 μm. Error bars are SEMs. (B and D–G) n = 3.