KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Chicken Polyclonal anti-GFP	Abcam	Cat# ab13970; RRID: AB_300798
Rat Monoclonal anti-mCherry	Thermo Fisher	Cat# M11217; RRID: AB_2536611
Goat Polyclonal anti-βgal	Bio-Rad	Cat# 4600–1409; RRID: AB_2307350
Mouse monoclonal anti-CALBINDIN-D-28K	Sigma	Cat# C9848; RRID: AB_476894
Rabbit Polyclonal anti-OTX2	Proteintech	Cat# 13497–1-AP; RRID: AB_2157176
Goat Polyclonal anti-Aldh1a1	R&D Systems	Cat# AF5869; RRID: AB_2044597
Rabbit Polyclonal anti-Sox6	Sigma	Cat# HPA001923; RRID: AB_1080065)
Guinea pig Polyclonal anti-Lmx1a	Gift from Yongchao C. Ma	N/A
Rabbit Polyclonal anti-Lmx1a	Millipore	Cat# AB10533; RRID: AB_10805970
Rabbit Polyclonal anti-CALBINDIN-D-28K	Millipore	Cat# AB1778; RRID: AB_2068336
Mouse Monoclonal anti-ALDH1A1	Sigma	Cat# SAB5300519
Rat Polyclonal anti-DAT	Santa Cruz Biotech	Cat# sc-32258; RRID: AB_627400
Mouse Monoclonal anti-RFP	Abcam	Cat# ab125244; RRID: AB_10973556
Sheep Polyclonal anti-TH	Pel-Freez	Cat# P60101–0; RRID: AB_461070
Chemicals, peptides, and recombinant proteins
Tamoxifen	Sigma	T5648
Experimental models: cell lines
PRX-B6 (C57BL/6N) (mouse ES cells)	The Jackson Laboratory	012448; RRID: IMSR_JAX:012448
Experimental models: mouse strains
Sox6-FSF-Cre	Poulin et al. 2018	N/A
Sox6-Cre	This paper	N/A
Sox6-CreERT2	This paper	N/A
Th-2A-Flpo	Poulin et al. 2018	N/A
Mouse: Tg(EIIa-cre)C5379Lmgd	The Jackson Laboratory	003724; RRID: IMSR_JAX:003724
Mouse: Slc17a6tm2(cre)Lowl	The Jackson Laboratory	016963; RRID: IMSR_JAX:016963
NSF	(Poulin et al., 2020b)	N/A
B6;129S6-Gt(ROSA)26Sortm8(CAG-mCherry,-EGFP) Dym/J	The Jackson Laboratory	029486; RRID: IMSR_JAX:029486
RC-Fela	A gift from S. Dymecki (Jensen et al. 2008)	N/A
Mouse: B6.Cg-Gt(ROSA)26Sortm65.2(CAG-tdTomato) Hze/J	The Jackson Laboratory	032864; RRID: IMSR_JAX:032864
Mouse: B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato) Hze/J	The Jackson Laboratory	007905; RRID: IMSR_JAX:007905
Mouse: B6;129-Tg(CAG-dre)1Afst/Mmucd	MMRRC	032246-UCD; RRID: MMRRC_032246-UCD
Oligonucleotides
Primers for Cre strains Cre-F: GCAGAACCTGAAGATGTTCGC	This paper	N/A
Primers for Cre strain Cre-R: ACACCAGAGACGGAAATCCATC	This paper	N/A
Primers for NSF F: CACCAAGACCAAGACCCTGT	This paper	N/A
Primers for NSF R: CCTTCAGCAGCTGGTACTCC	This paper	N/A
Primers for RC-Fela, RC-Frepe, RC-Ai9, RC-Ai65F -F: TGCAATACCTTTCTGGGAGTTC	This paper	N/A
Primers for RC-Fela, RC-Frepe, RC-Ai9, RC-Ai65F -R: AGCGGGAGAAATGGATATGAAG	This paper	N/A
Primers for RC-Fela, RC-Frepe, RC-Ai9, RC-Ai65F -R: TACCGTAAGTTATGTAACGCGG	This paper	N/A
Primers for Th-2A-Flpo F- TAAGACCCTGCTGATGGTTGG	This paper	N/A
Primers for Th-2A-Flpo R- CATAGGGCATTCCTGTGGTTTG	This paper	N/A
Primers for Th-2A-Flpo R- GCTTCACTGAGTCTCTGGCATC	This paper	N/A
Recombinant DNA
AAV5-hSyn-CreOn/FlpOn-EYFP	UNC	# AV8357
AAV8EF1α-CreOff/FlpOn-mCherry	gift from K. Deisseroth	#2466
AAV5-EF1α-DIO-mCherry	UNC	#AV4311B
AAVdj-hSyn-CreOff/FlpOn-eYFP	gift from K. Deisseroth	#987
AAV8-EF1α-CreOn/FlpOn-GCaMP6f	gift from K. Deisseroth	#2383
AAV8-EF1α-CreOff/FlpOn-GCaMP6f	gift from K. Deisseroth	#2385
Deposited data
RNaseq of GFP and mCherry cells of Sox6-FSF-Cre, Th-2A-Flpo, RC- Frepe SNc	This Paper	GEO: GSE185480
Software and algorithms
VS-ASW-S6	Olympus	https://www.olympus-lifescience.com/en/microscopes/virtual/vs120/
cellSens	Olympus	https://www.olympus-lifescience.com/en/software/cellsens/
Zen 2.3 and 3.3	Zeiss	https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html
MATLAB	MathWorks	https://www.mathworks.com/products/matlab.html
RStudio	RStudio	https://www.rstudio.com/
FastQC	Babraham Bioinformatics	https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
STAR	Dobin et al., 2013	https://github.com/alexdobin/STAR
DESeq2	Bioconductor	https://bioconductor.org/packages/release/bioc/html/DESeq2.html https://doi.org/10.18129/B9.bioc.DESeq2.
cutadapt	cutadapt	https://cutadapt.readthedocs.io/en/stable/ DOI: 10.14806
pheatmap	CRAN	https://cran.r-project.org/web/packages/pheatmap/index.html
GSEA	UCSD	https://www.gsea-msigdb.org/gsea/index.jsp
ImageJ	NIH	https://imagej.nih.gov/ij/