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. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: Nat Chem Biol. 2021 Nov 19;17(12):1271–1280. doi: 10.1038/s41589-021-00907-2

Extended Data Fig. 6: Y150 mediates 20(S)-OHC:Tmem97 binding.

Extended Data Fig. 6:

a, Homology model of mouse Tmem97 based on the cryo-EM structure of human EBP (PDB 6OHT) embedded in a phosphatidylcholine (POPC) bilayer. 20(S)-OHC docks in a central binding pocket.

b, Quantitative fluorescence analysis of probe 2 labeling with wild-type and Tmem97 and Y150 mutants shown in Fig. 5e, normalized to labeling of the wild-type Tmem97 construct in the presence of DMSO alone. Values are the average of 2 biological replicates.