TABLE 4.
Binding affinities of EMCV nt 680 to 786 RNA to eIF4GI mutants in the absence and in the presence of eIF4A
| eIF4GI mutant |
Kd (nM)
|
|
|---|---|---|
| −eIF4A | +eIF4A | |
| eIF4GI(643–1076) | 170 | 5 |
| eIF4GI(697–1076) | 400 | 6 |
| eIF4GI(722–1076) | 600 | 80 |
| eIF4GI(734–1076) | 700 | 650 |
| eIF4GI(746–1076) | 1,400 | 1,300 |
| eIF4GI(772–1076) | >6,000 | >6,000 |
| eIF4GI(697–969) | 2,300 | 40 |
| eIF4GI(697–949) | 2,800 | 400 |
| eIF4GI(697–941) | 3,000 | 3,000 |
| eIF4GI(697–869) | >8,000 | >8,000 |
| eIF4GI mut1 | 200 | 200 |
| eIF4GI mut4 | 120 | 30 |
| eIF4GI RRM1 | 1,800 | 400 |
| eIF4GI RRM2 | 480 | 10 |
| eIF4GI mut(I749T, R754I) | 1,000 | 200 |
| eIF4GI mut796-Ins8 | 1,200 | 400 |
| eIF4GI mut(R855A) | 500 | 8 |
| eIF4GI mut889-Ins6 | 800 | 5 |
| eIF4GI mut(R915I) | 1,700 | 300 |
| p97(NAT1) 62–330 | >4,000 | >4,000 |