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. 2021 Nov 8;9:741975. doi: 10.3389/fcell.2021.741975

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Copyright © 2021 Manfrevola, Martinez, Coutton, Rocco, Reynaud, Le Vern, Froment, Beauclair, Aubert, Pierantoni, Chianese and Guillou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative histograms and density plot showing the distribution of testicular germ cell populations, based on their DNA content, in WT and Ankrd31^–/– by flow cytometry. (A) FACS analysis of isolated WT male germ cells. (B) FACS analysis of isolated Ankrd31^–/– male germ cells. (C) Quantification analysis between WT and Ankrd31^–/– germ cell subpopulations isolated from n = 6 animals/each genotype. Data are expressed as percentage (%) of germ cells and reported as mean ± SEM, **p < 0.01.