TABLE 1.

Primer sequences and annealing temperatures.

Gene primers	Sequences 5′–3′	Tm (°C)
Ocln S	cctccacccccatctgacta	58
Ocln AS	cagcagcagccatgtactct
ZO-1 S	gcttctcttgctggccctaa	58
ZO-1 AS	tactctgagatgggggtggg
Cldn5 S	tgccggtgtcacagaagtac	56
Cldn5 AS	ttctccagctgccctttcag
CDH1 S	atcagctgccccgaaaatga	54
CDH1 AS	cttgaaggtcagcagcctga
Cx43 S	gtacccaacagcagcagact	54
Cx43 AS	ctgcttcaggtgcatctcca
RP18S S	gagactctggatgctaactag	56
RP18S AS	ggacatctaagggcatcacag
Ankrd31 S	catatatgctaatggtaccctacca	53
Ankrd31 AS	ccttgtaattagtaatttgccacag