FIGURE 2.

Characterization of tTA‐fused tetraspanins. (a) Cells were transfected with plasmids encoding tTA‐fused tetraspanins and TEVp. After 48 h, cells were lysed and subjected to western blotting. Upper and lower panels represent immunoblotting using anti‐TetR antibody and anti‐GAPDH antibody, respectively. The expected mass based on the amino acid sequences were as follows: CD63‐tTA, 63.2 kDa; CD81‐tTA, 63.4 kDa; CD9‐tTA, 63.0 kDa; tTA, 36.9 kDa. (b) Expression of NanoLuc under TRE3G promoter in HEK293T cells co‐expressing tTA‐fused tetraspanins and TEVp. As controls, plasmids encoding tTA without tetraspanin fusion and empty expression plasmid were used. Numbers above the bars indicate the fold increase in NanoLuc expression compared to the mock transfection. N = 3, mean ± SD