FIGURE 7.

NPM1mA induces miR‐19a‐3p expression by CTCF‐mediated transcription inhibition. (a) Leukemic cells were transfected with shRNA against NPM1 (shNPM1‐#3), and the expression of miR‐19a‐3p in the indicated cells and cell‐derived sEVs was detected by qRT‐PCR. (b) Illustration of the genomic location of miR‐19a in the host gene, MIR17HG. The CTCF‐binding motifs in the promoter region of MIR17HG are indicated. (c) CTCF binding to the promoter of MIR17HG was determined by performing ChIP assays. miR‐19a‐3p levels in immunoprecipitated samples were determined by qRT‐PCR and are shown as percentages with respect to the input sample (% input). (d) Transcriptional inhibition of MIR17HG by CTCF was determined by performing dual‐luciferase assays. (e) The enrichment of CTCF at the MIR17HG promoter in leukemic cells (treated with or without NPM1 shRNA) was detected by ChIP