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. 2021 Nov 22;10(13):e12168. doi: 10.1002/jev2.12168

FIGURE 9.

FIGURE 9

Leukaemia‐derived sEV‐related miR‐19a‐3p impairs CD8+ T cell antitumour function in vivo. The indicated OCI/AML3 cells (OCI/AML3, OCI/AML3‐NC, or OCI/AML3‐miR‐19a‐3p KD) were injected into HuHSC‐NSG mice. OCI/AML3, mock (untreated cells); OCI/AML3‐NC, NC (OCI/AML3 cells transfected with negative control vector); OCI/AML3‐miR‐19a‐3p KD, miR‐19a KD (OCI/AML3 cells transfected with shRNA targeting miR‐19a‐3p); OCI/AML3‐sEVs, sEVs (sEVs‐derived from OCI/AML3 cells). (a) Kaplan–Meier analysis of the survival curves of the mice in each group (n = 6). (b) The number of CD45+ human leukemic cells were determined by FCM. (c) Immature cells from the bone marrow were checked using Wright's stain. (d and e) Spleen and liver infiltration were analyzed by H&E staining (d) and IF for NPM1mA (e). (f) CD8+ T cells in bone marrow detected by IF for CD8. Proliferation (g), IL‐2 secretion (H), and IFN‐γ secretion (i) of bone marrow CD8+ T cells are shown. (j) SLC6A8 protein expression in CD8+ T cells was detected by western blotting. (k) Intracellular creatine in CD8+ T cells was determined using the Creatine Assay Kit (*p < 0.05; **p < 0.01; ***p < 0.001; n.s, not significant)