Boxplots depicting the distribution of normalized %CD63+ basophils for all samples of each nut and each concentration (0.001 to 1000 ng/mL). Allergic samples are depicted in red and non-allergic samples are depicted in blue (peanut allergic n=87, non-allergic n=46; hazelnut allergic n=48 non-allergic n=42, walnut allergic n=41, non-allergic n=35; cashew allergic n=50, non-allergic n=24; pistachio allergic n=45, non-allergic n=25). Dashed lines indicate the median values for each concentration. For each nut and concentration, the difference between allergic and non-allergic samples was statistically significant (p<0.05 Welch’s t-test), except for pistachio at 0.01 ng/mL concentration.