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Suppression of srp1-49 by a high dosage of STS1 and sts1 mutants. Plasmids containing STS1, sts1-11,12(E43G, I162T), and sts1ΔNLS1 under control of the GAL promoter were transformed into the srp1-49 strain. Resulting strains and a control SRP1 strain were streaked onto synthetic complete galactose (Gal) or glucose (Glu) plates lacking uracil and incubated for 3 days at 38°C. Vector pRS314 was used as a plasmid control.
