. 2021 Nov 22;16(11):e0260351. doi: 10.1371/journal.pone.0260351

Table 3. Testing the effect of age group, gender, and interaction between the two on the test metrics using one and two-way analysis of variance (ANOVA).

Test	Variables	(1)		(2)		(3)
		Age group		Gender		Age group		Gender		Age×Gender
		F-stat	p-value	F-stat	p-value	F-stat	p-value	F-stat	p-value	F-stat	p-value
Saccade–Random, Horizontal	Latency (s)	0.512	0.475	2.198	0.139	1.699	0.193	1.903	0.168	2.763	0.097
	Accuracy (%)	1.691	0.194	1.485	0.224	1.299	0.255	1.273	0.260	0.007	0.933
	Final accuracy (%)	1.211	0.272	0.160	0.689	0.305	0.581	0.025	0.875	2.287	0.131
	AUF (deg²/s)	1.626	0.203	0.020	0.888	1.322	0.251	0.002	0.967	0.033	0.856
	Peak velocity (25–30°/s)	0.001	0.970	2.871	0.093	0.001	0.982	2.318	0.131	0.028	0.868
Saccade–Random, Vertical	Latency (s)	8.416	0.004	0.000	0.986	12.430	0.000	0.003	0.958	5.773	0.017
	Accuracy (%)	0.037	0.847	0.905	0.342	0.048	0.827	0.940	0.333	0.008	0.928
	Final accuracy (%)	0.356	0.551	0.299	0.585	0.376	0.540	0.511	0.475	0.029	0.866
	AUF (deg²/s)	12.198	0.001	1.884	0.171	11.755	0.001	1.456	0.228	0.381	0.538
	Peak velocity (25-30deg/s)	0.002	0.967	0.491	0.485	0.070	0.792	0.000	0.993	1.112	0.294
Smooth Pursuit–Horizontal 0.1Hz	Velocity gain	0.078	0.779	0.888	0.346	0.064	0.801	0.962	0.327	0.062	0.804
	Asymmetry (%)	2.059	0.152	0.484	0.487	2.007	0.157	0.603	0.438	0.001	0.974
	Position gain	4.351	0.038	0.518	0.472	3.495	0.062	0.882	0.348	0.350	0.555
	Saccadic component (%)	0.010	0.921	2.055	0.152	0.058	0.811	2.509	0.114	2.070	0.151
Smooth Pursuit–Horizontal 0.75 Hz	Velocity gain	0.016	0.898	5.980	0.015	0.041	0.840	6.337	0.012	0.695	0.405
	Asymmetry (%)	0.143	0.706	0.710	0.400	0.391	0.532	0.830	0.363	1.113	0.292
	Position gain	13.658	0.000	2.349	0.126	14.923	0.000	2.729	0.099	0.671	0.413
	Saccadic component (%)	2.878	0.090	0.847	0.358	1.414	0.235	0.464	0.496	1.312	0.253
Smooth Pursuit–Vertical 0.1 Hz	Velocity gain	2.122	0.146	0.009	0.924	1.506	0.220	0.035	0.853	0.003	0.958
	Asymmetry (%)	1.871	0.172	1.567	0.211	1.757	0.186	2.164	0.142	0.061	0.805
	Position gain	16.354	0.000	0.086	0.770	14.096	0.000	0.270	0.604	0.057	0.811
	Saccadic component (%)	4.032	0.045	3.654	0.057	3.250	0.072	3.133	0.077	0.002	0.964
Smooth Pursuit–Vertical 0.75 Hz	Velocity gain	0.500	0.480	3.727	0.054	0.179	0.672	3.313	0.069	0.218	0.641
	Asymmetry (%)	6.709	0.010	2.177	0.141	3.775	0.053	1.411	0.236	1.727	0.190
	Position gain	4.317	0.038	0.000	0.983	6.335	0.012	0.000	0.990	2.995	0.084
	Saccadic component (%)	5.479	0.020	11.492	0.001	3.486	0.063	10.010	0.002	0.349	0.555
Predictive Saccades	Percentage predicted (%)	35.909	0.000	0.043	0.835	28.757	0.000	0.057	0.812	1.515	0.219
Antisaccades	Error rate (%)	4.780	0.029	2.799	0.095	0.060	0.806	2.281	0.132	3.649	0.057
Visual Reaction Time	Latency (ms)	0.834	0.362	12.277	0.001	2.041	0.154	0.080	0.778	3.168	0.076
Auditory Reaction Time	Latency (ms)	0.018	0.894	29.000	0.000	0.815	0.367	27.972	0.000	0.593	0.442
Saccade and Reaction Time–Saccade metrics:	Latency (ms)	0.547	0.460	0.406	0.524	0.550	0.459	0.370	0.543	0.067	0.795
	Accuracy (%)	0.822	0.365	1.623	0.203	0.371	0.543	1.317	0.252	0.348	0.556
	Final accuracy (%)	0.008	0.927	1.696	0.193	0.007	0.935	1.765	0.185	0.071	0.790
Saccade and Reaction Time–Motor response:	Latency mean (s), Left	0.020	0.887	0.190	0.664	0.019	0.892	0.103	0.749	0.004	0.949
Saccade and Reaction Time–Motor response:	Latency mean (s), Right	0.007	0.932	0.060	0.807	0.000	0.995	0.087	0.768	0.030	0.864

The t-test-based results examining the effect of age and gender are not reported here. However, these results are consistent with those of one-way ANOVA.