Table 1. S19-GFP vaccination protocols of 25 crossbred heifers of 8–11 months of age.

Group	Vaccination route	N° of animals	Dosesa	GST-GFP boostb	Week of boost
A	Subcutaneousc	5	S19-GFP	None	None
B	Subcutaneous	10	S19-GFP + GFPd	150 μg	7
C	Subcutaneous	5	S19-GFP	150 μg	7
D	Conjunctivale	5	S19-GFP	150 μg	7

^aIndividual S19-GFP vaccine suspensions contained 5×10⁹ CFU.

^bIndividual GST-GFP boosting doses were always diluted in 2 mL of a 1% sterile calcium alginate suspension and administered subcutaneously.

^cSubcutaneous vaccine was prepared in 2 mL of 0.1M sterile PBS.

^dWhen required the 300 μg of GFP given simultaneously with the S19-GFP was directly dissolved in the corresponding vaccine suspension.

^eConjunctival vaccine was prepared in 70 μL of 0.1M sterile PBS.