Appendix 4—figure 3. Convergence for reporter pairs for low gene activity.

In each case, the mean nascent mRNA level is 0.5, the mean mature mRNA level of 5, and the mean protein level is 500. The simulated genes are constitutive and noise is on all parameters except for ${\delta }_M=1$. Each graph shows the convergence of 600 individual reporter simulations, for each combination of reporters from nascent mRNA, mature mRNA and protein. Each reporter simulation is from $10,000$ samples, with the reporter estimates calculated at intervals of 100. The noise on $K_N$ has squared coefficient of variation equal to 0.2, which should be identified by both the nascent-mature reporter and mature-mature dual reporter. As in Figure Convergence of Pathway and Dual Reporters, the mature-protein reporter should converge to an estimate of approximately 0.2315. Nascent-nascent and protein-protein reporters identify combined noise on more parameters, so do not converge to 0.2. The lower graph shows each of nascent-mature, mature-mature, mature-protein and nascent-protein in the same plot for direct comparison.