Appendix 5—table 1. A comparison of the pathway-reporter method and dual-reporter method for constitutive gene expression with Erlang-distributed maturation times (Case (1)A).

Here, PR (NP) gives the results of the nascent and protein pathway reporters, PR (MP) gives the results of the mRNA and protein reporters, while DR (Mat) gives the results of the dual reporters calculated from the mature mRNA. The maturation time $T_{\mathrm{mat}}$ is chosen to be Erlang distributed with mean length $1/30,0.05$, and 0.1, respectively. We consider the rate parameters for the remaining exponentially distributed times to be constant, so that there is no extrinsic noise. The pathway-reporters correctly identify the zero extrinsic noise contribution.

Parameters				Simulation
(r)1-6 $K_N$	$T_{\mathrm{mat}}(mean)$	$K_P$	${\delta }_P$	Pr(NM)	Pr (MP)	Pr (NP)	DR (Mat)
300	${\displaystyle 0.0\dot{3}}$	${\displaystyle 0.0\dot{6}}$	0.1	$0.00\pm 0.001$	$0.00\pm 0.0001$	$0.00\pm 0.0003$	$0.00\pm 0.0001$
200	0.05	1	0.1	$0.00\pm 0.001$	$0.00\pm 0.0001$	$0.00\pm 0.0004$	$0.00\pm 0.0001$
100	0.1	2	0.1	$0.00\pm 0.001$	$0.00\pm 0.0002$	$0.00\pm 0.0001$	$0.00\pm 0.0004$