Appendix 5—table 6. A comparison of the pathway-reporter method and dual-reporter method for the multiscale model with a fixed duration of maturation (Case (3)B).

Here the maturation time, $T_{\mathrm{mat}}$, is set to 0.05. For each of the parameters ${\lambda }_1,{\mu }_1,K_P,{\delta }_P$, we selected a scaled ${\displaystyle \mathrm{B}\mathrm{e}\mathrm{t}\mathrm{a}(5,6)}$ distribution, with squared coefficient of variation ${\eta }^2=0.1$; the scaling is chosen in each case to achieve a mean value equal to the parameter value. The parameter ${\lambda }_2=K_N$ is given the noise distribution ${\displaystyle \mathrm{B}\mathrm{e}\mathrm{t}\mathrm{a}(3,6)}$, which has a slightly higher coefficient of variation ${\eta }^2=0.2$. The extrinsic noise values given by pathway reporters match well with those obtained by dual reporters.

Mean					Simulation
(r)1-5 ${\lambda }_1$	${\mu }_1$	${\lambda }_2=K_N$	$K_P$	${\delta }_P$	Pr (MP)	Pr (NP)	DR (Mat)
2	2	400	2	0.1	$0.18\pm 0.02$	$0.17\pm 0.04$	$0.15\pm 0.02$
4	20	1210	2	0.1	$0.30\pm 0.05$	$0.30\pm 0.12$	$0.28\pm 0.05$