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. 2021 Nov 23;19:309. doi: 10.1186/s12916-021-02184-1

Table 2.

Saliva antibody conversion rates between the first and last study visit

Antibody Antigen Increaseda
N (%)
Decreasedb
N (%)
Maintainedc
N (%)
Fold change: < 4 ≥4 Total < 4 ≥4 Total -
IgM N CTd 207 (13.6%) 0 (0%) 207 245 (16.1%) 0 (0%) 245 1066 (70.2%)
N FL 241 (15.9%) 1 (0.06%) 242 344 (22.6%) 1 (0.06%) 345 931 (61.3%)
RBD 192 (12.6%) 0 (0%) 192 212 (13.9%) 0 (0%) 212 1114 (73.4%)
S 223 (14.7%) 0 (0%) 223 243 (16.0%) 0 (0%) 243 1052 (69.3%)
S2 268 (17.6%) 2 (0.1%) 270 340 (22.4%) 2 (0.13%) 342 906 (59.7%)
Global 3 (0.2%) 0 (0.0%) -
N FL only 1 (0.1%) - -
IgA N CT 524 (34.5%) 20 (1.3%) 544 619 (40.8%) 27 (1.7%) 646 328 (21.6%)
N FL 546 (35.9%) 15 (1.0%) 561 656 (43.2%) 26 (1.7%) 682 275 (18.1%)
RBD 461 (30.4%) 5 (0.3%) 466 551 (36.3%) 7 (0.4%) 558 494 (32.5%)
S 442 (29.1%) 3 (0.2%) 445 529 (34.8%) 6 (0.4%) 535 538 (35.4%)
S2 481 (31.7% 8 (0.5%) 489 627 (41.3%) 19 (1.2%) 646 383 (25.2%)
Global 36 (2.3%) 1 (0.1%) -
N FL only 8 (0.5%) - -
IgG N CT 566 (37.3%) 14 (0.9%) 580 509 (33.5%) 14 (1.0%) 523 415 (27.3%)
N FL 586 (38.6%) 15 (1.0%) 601 561 (36.9%) 18 (1.3%) 579 338 (22.3%)
RBD 439 (28.9% 2 (0.1%) 441 419 (27.6%) 5 (0.3%) 424 653 (43.0%)
S 418 (27.5%) 3 (0.2%) 421 376 (24.8%) 5 (0.3%) 381 716 (47.2%)
S2 504 (33.2%) 6 (0.4%) 510 500 (32.9%) 12 (0.8%) 512 496 (32.7%)
Global 26 (1.7%) 4 (0.3%) -
N FL only 9 (0.6%) -
Total 49 (3.2%) 0 (0.0%) -
N FL only 13 (0.9%) - -

aThe number (N) of subjects who increased antibody levels was calculated for each isotype/antigen pair, per Ig isotype, and globally, out of the 1518 individuals in whom two samples were available with ≥6 days of difference (see also Fig. 1). Individuals who increased antibody levels ≥4-fold change (FC) for at least one isotype/antigen were considered antibody positive. The total saliva antibody conversion rate (% in bold) was calculated as the proportion of positive individuals

bA decrease in antibody levels ≥4 FC was interpreted as negativization for any given isotype/antigen pair. Within an individual, complete antibody reversion was considered only if the antibody levels decreased ≥4 FC for all the isotype/antigen pairs

cIndividuals who maintained antibody levels between visits are computed for comparison

dN nucleocapsid, CT C-terminus end, FL full-length, RBD receptor binding domain of spike (S). Antibody conversion for N FL is shown separately as representative of potential cross-reactivity with endemic human coronaviruses