CLU was significantly downregulated in seminoma according to database and clinical tissues
(A) Data downloaded from Oncomine were redrawn by GraphPad software. (B) qRT-PCR analyses were performed with 38 seminoma tissues and 12 normal testes to explore the mRNA expression of CLU. (C) Western blot analyses were performed with 10 pairs of seminoma tumor tissues and adjacent normal tissues to explore the protein expression of CLU. (D) IHC analyses were further used to identify the expression difference. Scale bar: 100 μm. (E) Representative stains of seminoma tissues by IHC, 1 (negative), 2 (weak brown), 3 (moderate brown), 4 (strong brown). Data are represented as mean ± SD. Scale bar: 100 μm, ∗∗∗∗p < 0.0001.