Figure 2.

CLU curbed Tcam-2 cells proliferation in vitro and in vivo through arresting cell cycle in G1 phase

(A) Stable CLU-knockdown and -overexpression cell lines were established successfully. (B) The optical density (OD) values detected in the CCK-8 proliferation assay were performed after 1–4 days seeding, and higher OD values indicate higher cell viability. (C) Colony formation was photographed 2 weeks after seeding and quantified by ImageJ software. (D) Xenografts in mice in vivo and their ki-67 staining (E), volumes (F), and weights (G). (H) Flow cytometric cell cycle analysis of shCLU, oeCLU, and NC cells showed the percentage of cells in G1 or G2+S phase. Data are represented as mean ± SD. ∗p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Scale bar: 100 μm.