Figure 4.

The expression of ECM-associated COL15A1 and COL7A1 was significantly changed with CLU knockdown or overexpression

(A) The most enriched GO terms based on the RNA-seq data of oeCLU and oe-NC groups. (B) Data downloaded from TCGA were submitted to GSEA and extracellular matrix organization was also significantly enriched in samples with higher CLU expression. (C) 21 and 115 upregulated ECM-associated genes from GO and GSEA terms were overlapped. COL15a1, COL7A1, and VCAN were screened out. (D) The co-expression of CLU with COL15a1, COL7A1, and VCAN based on the database: GEPIA. (E) The verification of co-expression relationship with 50 seminoma tissues. (F and G) mRNA and protein expression of COL15a1, COL7A1, and VCAN in pretreated Tcam-2 cells. Data are represented as mean ± SD. ∗p < 0.05.