Figure 7.

CLU interacted with MEF2A to upregulate COL15a1

(A) Jaspar database was used to estimate the potential TFs that could bind to the promoter of COL15a1, and ChIP assay was performed to prove this in oeCLU and NC groups. (B) Luciferase reporter assay was performed in shMEF2A cells to detect the luciferase activity. The mRNA expression of COL15a1 was then explored with qRT-PCR in shMEF2A, oeCLU, and oeCLU + shMEF2A groups. (C) The mutated promoter of COL15a1 was constructed. The luciferase activity of MEF2A was then detected in WT and mutant groups with cells transfected by shCLU, shMEF2A, oeCLU + shMEF2A, and NC. (D) Immunoprecipitation assay was performed with total, nucleus, and cytoplasm protein respectively to study the directly interaction between CLU and MEF2A. (E) Co-localized IF staining displayed the distribution of CLU and MEF2A. (F) The analysis of COL15a1 expression in seminoma based on UALCAN database. (G) The mechanism of CLU in inhibiting testicular seminoma metastasis.