Figure 1.

lncRNA-DAW was driven by a liver-specific super-enhancer

(A) The H3K27ac ChIP-seq data of lncRNA-DAW (LINC01430) locus in a panel of normal human tissues. The yellow region indicates the gene locus of lncRNA-DAW. (B) The ChIP-seq data of super-enhancer histone markers of lncRNA-DAW locus in normal hepatocytes. (C) The RNA-seq data from NCBI showed that lncRNA-DAW (LINC01430) was highly expressed in human liver tissues. (D) The RNA levels of lncRNA-DAW were measured across a panel of adult human tissues by using qRT-PCR assays. (E) Cellular localization of lncRNA-DAW. The relative levels of cytoplasmic RNA transcript β-actin, nuclear RNA transcript U1, and lncRNA-DAW from different cell compartments were assessed by qRT-PCR assays. The total levels for each gene were considered as 100% and results were presented as a relative percentage of total levels (n = 4). (F) Compared with LO2 cells, lncRNA-DAW was upregulated in most of the HCC cell lines. (G) The expression levels of lncRNA-DAW in 50 pairs of HCC and adjacent normal tissues were determined by qRT-PCR assays.