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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Kidney Int. 2021 Jul 1;100(6):1179–1184. doi: 10.1016/j.kint.2021.05.040

Figure 1. Intersectional recombinase approach with orthogonal recombinases.

Figure 1.

(a) Intersectional lineage tracing is achieved by using two orthogonal recombinases driven by promoters of two different genes in parallel. (b) The Ai65 reporter allele has two STOP cassettes flanked by two FRT or loxP sites. Simultaneous expressions of Flp and Cre remove two STOP cassettes to express tdTomato. (c) RC::Fela reporter allele is a dual-color reporter. Flp expression turns on only GFP expression, and concurrent expression of Flp and Cre turns on only β-galactosidase. Single Cre expression does not label a cell.