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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Kidney Int. 2021 Jul 1;100(6):1179–1184. doi: 10.1016/j.kint.2021.05.040

Figure 3. Genomic lineage tracing with CRISPR-Cas9 based genetic scarring and the CARLIN system.

Figure 3.

(a) Genomic lineage tracing with CRISPR-Cas9 based genetic scarring system. Intrinsic DNA barcodes are scarred by Cas9 with deletion or insertion to render them unique (left). As a result, lineage trees are recovered based on shared scars (right). (b) CRISPR array repair lineage tracing (CARLIN). CARLIN adopted “Tet-ON” system with the reverse tetracycline-controlled transactivator (rtTA) for induction of scars (top). Cas9 expression is induced by doxycycline (Dox) treatment. Ten gRNAs are also constitutively expressed (middle). Cas9 protein and gRNA generate genetic scars in each target sequence on target array (CARLIN array). CARLIN array is expressed as a 3’ UTR of a GFP transcript, enabling capture in scRNA-seq (bottom).