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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: J Neurosci Methods. 2021 Oct 8;365:109384. doi: 10.1016/j.jneumeth.2021.109384

Fig. 2. Consecutive food preference testing in 14-week old C57BL6 mice.

Fig. 2.

Mice were raised on standard chow (Teklad 8664, 3.1 kilocalories per gram). Concurrent intake of two novel test foods (BioServ F3028, 3.35 kilocalories per gram and Bioserv F3156, 3.74 kilocalories per gram) was measured during 4, consecutive 24-hour test periods. (A): Body weight at the start of each test day in female mice (n=8, ●). Results were compared using repeated measures one-way ANOVA [F(1.962,13.73)=1.809, p=0.2011] with Bonferroni correction for multiple comparisons. Means ±s.e.m. (B): Body weight at the start of each test day in male mice (n=8, ■). Results were compared using repeated measures one-way ANOVA [F(2.267,15.87)=25.00, p<0.0001] with Bonferroni correction for multiple comparisons (*p≤0.05, **p≤0.01, ***p≤0.001). Means ±s.e.m. (C): Daily consumption of test foods in female mice (n=8, ●). Results were compared using repeated measures two-way ANOVA [test food: F(1,14)=921.0, p<0.0001; test day: F(2.422,33.91)=9.045, p=0.0004; test food × test day: F(3,42)=5.661, p=0.0024] with Bonferroni correction for multiple comparisons (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). Means ±s.e.m. (D): Daily consumption of test foods in male mice (n=8, ■). Results were compared using repeated measures two-way ANOVA [test food: F(1,14)=520.5, p<0.0001; test day: F(2.390,33.46)=4.940, p=0.0096; test food × test day: F(3,42)=5.322, p=0.0034] with Bonferroni correction for multiple comparisons (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). Means ±s.e.m. (E): Estimated daily energy intake in female mice (n=8, ●). Results were compared using repeated measures one-way ANOVA [F(2.128, 14.89)=15.87, p=0.0002] with Bonferroni correction for multiple comparisons (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). Means ±s.e.m. (F): Estimated daily energy intake in male mice (n=8, ■). Results were compared using repeated measures one-way ANOVA [F(2.272,15.91)=6.332, p=0.0078] with Bonferroni correction for multiple comparisons (*p<0.05). Means ±s.e.m.