Figure 7. Kidney function, and KLF15 and PPARA expression levels correlate in human kidneys.

(a) Co-immunofluorescence for KLF15 and AQP1, with co-staining for Phaseolus vulgaris Erythroagglutinin (PHA-E) and DAPI in a control subject and a CKD patient exposed to AA. Scale bars = 100μm. (b) Analysis of correlation between KLF15 and PPARA expression levels from previously reported expression data³⁵ in healthy subjects. (c) Multiple variable Pearson correlation analysis of GFR, and KLF15, PPARA, and CPT1A expression levels using previously reported tubulointerstitial expression data from 59 healthy, HTN and DN samples.³⁵ Pearson r correlation coefficients and p-values are shown for each pairwise comparison. (d) Correlation between eGFR, and KLF15, PPARA, and CPT1A gene expression using expression array data from the tubulointerstitial compartment of 164 human kidney disease patients.³⁵ Each dot in the graph and column in the heatmap represents an individual patient, ranked by eGFR. (e) KLF15 and PPARA gene expression in the 164 patients categorized by eGFR. *p<0.05, **p<0.01, ***p<0.001, one-way ANOVA with Dunnett’s multiple comparisons test.