Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 22;12:6752. doi: 10.1038/s41467-021-27020-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Michaelis–Menten plot and kinetics parameters of the AtDAO1 enzyme for IAA-Glu (n = 3). Error bars, SE. b in vivo conversion of IAA-Asp-DM to IAA. Endogenous and [D₂]IAA-Asp and [D₂]IAA were measured after incubation of 8-d-old WT and ilr1 ill2 iar3 ill3 (ilr1/ill) quadruple mutants with 1 μM [D₂]IAA-Asp-DM for 6 h. The values shown are the means ± SD (***P < 0.001, ns: not significant, two-tailed Student’s t test, n = 4). c Phenotypes of dao1-1- and AtDAO1-overexpressing (35 S::AtDAO1) plants grown for 6 days with 1 μM IAA-Asp-DM. Scale bar, 10 mm. d Effects of IAA-Glu on root growth in dao1-1, dao1 dao2, 35 S::AtDAO1, iar3 dao1-1, and ilr1 dao1-1 mutants. Root length (6-d-old) is shown as the percentage of that in mock-treated plants (100%). The different letters represent statistical significance at P < 0.001 (Tukey’s HSD test, n = 20). e The levels of IAA metabolites after treatment with IAA-amino acid diesters. WT, dao1-1, and GFP-AtDAO1-overexpressing dao1-1 mutants (7-d-old) were incubated for 20 h with the compounds. The values shown are the means ± SD (***P < 0.001, two-tailed Student’s t test, n = 3).