Fig. 5. ILR1/ILL enzymes are involved in IAA release from IAA-Asp and IAA-Glu in Arabidopsis.
a Sensitivity of ilr1/ill mutants (Ws background) to IAA-Glu-DM. Root length (6-d-old) is shown as the percentage of that in mock-treated plants (100%). The different letters represent statistical significance at P < 0.001 (Tukey’s HSD test, n = 34‒42). b Expression patterns of ILR1-GFP and ILR1-GUS fusion proteins under the ILR1 promoter. At least five plants from each line were examined. Scale bars, 1 mm. c, d Hypocotyl length and lateral root number of 8-d-old ilr1 ill2 iar3 ill3 quadruple mutants. The different letters represent statistical significance at P < 0.001 (Tukey’s HSD test, b–c in Fig. 5d: P = 0.016). e Cotyledon area of dao1-1, dao1-1 ilr1, and dao1-1 ilr1 iar3 mutants. The plants were grown on vertical GM plates for 8 days. Scale bar, 5 mm. The different letters represent statistical significance at P < 0.001 (Tukey’s HSD test).