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. 2021 Nov 22;12:6752. doi: 10.1038/s41467-021-27020-1

Fig. 6. ILR1 hydrolyzed IAA amino acid conjugates to produce oxIAA.

Fig. 6

a HPLC chromatogram of the reaction products of the recombinant GST-ILR1 enzyme. b Lineweaver-Burk plot and kinetics parameters of GST-ILR1 for oxIAA-Glu as the substrate (n = 4). c The endogenous levels of oxIAA and oxIAA-Glu in WT, ilr1-1 (Ws, EMS mutant), ilr1 (Col, SAIL_631_F01) and pILR1::ILR1-GFP in ilr1 (Col) complementation lines. Seven-day-old plants were treated with 0.5 μM IAA-Asp-DM or IAA-Glu-DM for 20 h. The values shown are the means ± SD (n = 3). The different letters represent statistical significance at P < 0.001 (Tukey’s HSD test). d The endogenous levels of oxIAA-Asp, oxIAA-Glu, and oxIAA in WT and ilr1 (Col) seedlings. Seven-day-old plants were treated with 0.5 μM IAA for 24 h. The values shown are the means ± SD (two-tailed Student’s t test, n = 3).