Fig. 8. MIAT inhibited tumor growth in vivo and the hypotheses of the ceRNA.

A, B sh-NC and MIAT-shRNA of TPC-1 cells stably injected subcutaneously into different groups of nude mice. Tumor volume was measured with Vernier calipers every 4 days, and calculated as a (length) × b² (width). Five weeks after the injection, mice were photographed and killed, tumor growth curves were obtained. Error bars indicate mean ± SE/SD, compared with the control group. C qRT-PCR analyses of tumor issues showed that the mRNA and protein expression of the relative components decreased. D Molecular mechanism hypotheses of MIAT on the biological behavior of papillary thyroid carcinoma cells. MIAT and hsa-mir-150-5p were encoded by 22q12.1 and 19q13.33, respectively. Upregulation of MIAT combined with more miRNA (e.g., hsa-mir-150-5p etc.), resulting in the decrease of free hsa-mir-150-5p, the number of proteins encoded by EZH2 is relatively increased, as well as the EZH2/PRC2 complex. The complex can combine with the promoter H3K27me3 structure of tumor suppressor genes such as p15, and inhibit the expression of tumor suppressor genes, so as to promote the proliferation, invasion and metastasis of PTC cells. *P < 0.05, **P < 0.01, ***P < 0.001 compared with control cells, n.s. non-significant, using two-tailed t-test analysis.