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. 2021 Nov 23;7:365. doi: 10.1038/s41420-021-00712-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A circKDM4C and KDM4C mRNA levels in UM-UC3 and T24 cells treated with si-circKDM4C or negative control (siNC). B Effects of circKDM4C silencing on cell migration capability were assessed by wound-healing assays. Scale bar = 500 μm. C, D Effects of circKDM4C silencing on cell migration and invasion capabilities were determined by transwell migration and Matrigel invasion assays in UM-UC3 (C) and T24 (D) cells, respectively. Scale bar = 200 μm. E circKDM4C and KDM4C mRNA levels in UM-UC3 and T24 cells after transfection of circKDM4C-overexpression plasmid or the corresponding vector plasmids. F Effects of circKDM4C overexpression on cell migration capability were assessed by wound-healing assays. Scale bar = 200 μm. G, H Effects of circKDM4C overexpression on cell migration and invasion capabilities were determined by transwell migration and Matrigel invasion assays in UM-UC3 (G) and T24 (H) cells. Scale bar = 200 μm. I Effects of circKDM4C silencing on expression levels of EMT-associated proteins were detected by western blotting analysis. J Live imaging showed metastasis focus on forelimb (top) and perisplenic lymph node (bottom). K H&E staining of perisplenic lymph-node metastasis focus. Scale bar = 100 μm. Data are expressed as the means ± SD for n = 3. **P < 0.01; ***P < 0.001.