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. 2021 Nov 23;7:365. doi: 10.1038/s41420-021-00712-9

Fig. 4. miR-200b-3p and miR-200c-3p suppress BCa cell invasion and metastasis via targeting ZEB1.

Fig. 4

A–D Effects of miR-200b-3p/miR-200c-3p mimics (A, B) and miR-200b-3p/miR-200c-3p inhibitors (C, D) on cell migration and invasion capabilities were assessed through transwell migration and Matrigel invasion assays in UM-UC3 and T24 cells, respectively. Scale bar = 200 μm. E, F MiR-200b-3p and miR-200c-3p expression levels in different pathologic-stage BCa from the TCGA database. G, H Kaplan–Meier survival analysis indicated better survival probability in BCa patients with higher miR-200b-3p (G) or miR-200c-3p (H) expression levels (TCGA database). I Schematic graph of human ZEB1 3′ UTR and conserved miR-200bc-3p-site sequences. ORF: open-reading frame. J Western blotting assays indicated that miR-200b-3p/miR-200c-3p can downregulate the expressions of ZEB1, N-Cadherin, and vimentin, while upregulating E-Cadherin in BCa cells. Data are expressed as the means ± SD for n = 3. ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001.