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. 2021 Nov 23;7:365. doi: 10.1038/s41420-021-00712-9

Fig. 5. circKDM4C sponges miR-200b-3p and miR-200c-3p to enhance ZEB1 expression and promote BCa cell migration and invasion.

Fig. 5

A–D Transwell migration and Matrigel invasion assays for UM-UC3 (A, B) and T24 (C, D) cells after circKDM4C knockdown alone or cotransfected with miR-200b-3p or miR-200c-3p inhibitors. Scale bar = 200 μm. E Wound-healing assays of UM-UC3 and T24 cells. Scale bar = 500 μm. F Western blotting assays showed that miR-200b-3p/miR-200c-3p inhibitors partly reversed the decrease of ZEB1 expression induced by circKDM4C knockdown in BCa cells. G Quantification of ZEB1 levels. H Expression levels of ZEB1 in different pathologic-stage BCa from the TCGA database. I Kaplan–Meier survival analysis indicated poorer survival probability in BCa patients with higher ZEB1 expression. InNC: inhibitor NC; InB/InC: miR-200b-3p/miR-200c-3p inhibitor. Data are expressed as the means ± SD for n = 3. ns, no significance; **P < 0.01; ***P < 0.001.