Fig. 2.

Expression of the PPARα endocannabinoid receptor in the cerebellum. (a, b) Sagittal section of the vermis of a WT mouse (a) and confocal image with tridimensional projection (b) showing the co-localization of PPARα (green) in Purkinje cells (Cb28k, red) at P30. (c) Micrographs showing the expression of the PPARα (red), CB1 receptor (green), and Purkinje cells (Cb28k, blue) in WT and PCD mice throughout the postnatal cerebellar development (P7, P15, P17, P22, P30); PPARα expression can be observed in the three cerebellar layers at all postnatal ages. The CB1 receptor is expressed in the molecular layer from P7 onwards. In addition, from P15 onward, the CB1 receptor begins to be expressed around Purkinje cell somata (Cb28k positive), leading to the “Pinceaux formation.” (d) Quantification of the percentage of Purkinje cells expressing PPARα at different time points of the postnatal cerebellar development; note that this value is lower in PCD mice than in WT from P15 to P22. (e) Quantification of the percentage of PCD’s Purkinje cells expressing PPARα at P30 after different OEA dosages; OEA administration leads to an increase in the number of Purkinje cells expressing PPARα in the three different treatments at doses of 5 and 10 mg/kg. Data are represented as mean ± SEM; n = 4 each experimental group; Student’s t test for (d); one-way ANOVA followed by Dunnett’s post hoc test for (e); *p < 0.05; **p < 0.01