Fig. 9.

In vitro effect of OEA on WT and Ccp1-KO microtubule dynamics of MEFs.(a) Analyses of WT and Ccp1-KO microtubule growth and shrinking rates; OEA administration affects both parameters and experimental groups in a dose-dependent manner, although a higher OEA concentration is required in Ccp1-KO cells. (b) Analyses of WT and Ccp1-KO static microtubule curvature; OEA administration decreased Ccp1-KO microtubule curvature but did not affect WT curvature. (c) Analyses of WT and Ccp1-KO microtubule trajectory curvature; OEA did not affect trajectory curvature in any of the two genotypes. (d, e) Examples of WT and Ccp1-KO microtubule curvature (d) and trajectory (e) in control and OEA-administered cells (1.0 μM) and the corresponding graphical representation. Data are represented as mean ± SEM; n = 3 embryos per experimental group; a.u. = arbitrary units; one-way ANOVA followed by Dunnett’s post hoc test for (a–c); *p < 0.05