Table 2.
Associations of DNAm algorithms and frailty index with mortality
| Mortality |
Subset I (n=741) HR (95% CI) |
Subset II (n=1030) HR (95% CI) |
Overall (n=1771, meta-analysis) HR (95% CI) |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Cases | Model 1 | Model 2 | Cases | Model 1 | Model 2 | Cases | Model 1 | Model 2 | |
| All-cause | |||||||||
| AgeAccelPheno (per SD) | 194 | 1·39 (1·20-1·62) | 1·35 (1·16-1·57) | 264 | 1·37 (1·20-1·56) | 1·29 (1·13-1·48) | 458 | 1·38 (1·25-1·52) | 1·32 (1·19-1·46) |
| AgeAccelGrim (per SD) | 194 | 1·50 (1·31-1·73) | 1·39 (1·18-1·63) | 264 | 1·68 (1·48-1·91) | 1·56 (1·34-1·81) | 458 | 1·60 (1·46-1·75) | 1·47 (1·32-1·64) |
| MRscore-8CpGs (per SD) | 194 | 1·87 (1·55-2·26) | 1·65 (1·33-2·04) | 264 | 1·99 (1·68-2·36) | 1·82 (1·47-2·24) | 458 | 1·94 (1·71-2·20) | 1·73 (1·49-2·01) |
| FI (%; per SD) | 194 | 1·37 (1·20-1·56) | 1·35 (1·18-1·55) | 264 | 1·32 (1·18-1·48) | 1·28 (1·14-1·44) | 458 | 1·34 (1·23-1·46) | 1·31 (1·20-1·43) |
| Cancer | |||||||||
| AgeAccelPheno (per SD) | 60 | 1·38 (1·04-1·85) | 1·36 (1·02-1·81) | 78 | 1·18 (0·93-1·51) | 1·11 (0·87-1·42) | 138 | 1·26 (1·05-1·52) | 1·21 (1.00-1·46) |
| AgeAccelGrim (per SD) | 60 | 1·56 (1·23-1·99) | 1·32 (0·95-1·83) | 78 | 1·49 (1·18-1·89) | 1·34 (1·01-1·78) | 138 | 1·52 (1·29-1·80) | 1·33 (1·08-1·65) |
| MRscore-8CpGs (per SD) | 60 | 2·29 (1·65-3·19) | 1·79 (1·21-2·65) | 78 | 1·88 (1·38-2·58) | 1·72 (1·17-2·53) | 138 | 2·07 (1·65-2·59) | 1·76 (1·33-2·31) |
| FI (%; per SD) | 60 | 1·36 (1·07-1·73) | 1·29 (1.00-1·66) | 78 | 0·99 (0·79-1·25) | 0·98 (0·77-1·24) | 138 | 1·16 (0·98-1·37) | 1·11 (0·94-1·32) |
| CVD | |||||||||
| AgeAccelPheno (per SD) | 53 | 1·37 (1·03-1·83) | 1·31 (0·98-1·77) | 80 | 1·62 (1·28-2·06) | 1·57 (1·23-2·01) | 133 | 1·51 (1·26-1·82) | 1·46 (1·21-1·77) |
| AgeAccelGrim (per SD) | 53 | 1·20 (0·91-1·57) | 1·09 (0·81-1·47) | 80 | 1·76 (1·39-2·23) | 1·75 (1·31-2·33) | 133 | 1·49 (1·24-1·78) | 1·39 (1·13-1·71) |
| MRscore-8CpGs (per SD) | 53 | 1·71 (1·18-2·48) | 1·65 (1·09-2·48) | 80 | 2·09 (1·53-2·86) | 2·12 (1·45-3·12) | 133 | 1·93 (1·52-2·45) | 1·89 (1·42-2·50) |
| FI (%; per SD) | 53 | 1·49 (1·16-1·91) | 1·47 (1·14-1·90) | 80 | 1·53 (1·24-1·89) | 1·51 (1·22-1·88) | 133 | 1·51 (1·29-1·78) | 1·49 (1·27-1·76) |
Abbreviations: HR, hazard ratio; CI, confidence interval; SD, standard deviation; AgeAccelPheno, DNA methylation phenotypic age acceleration; AgeAccelGrim, DNA methylation GrimAge acceleration; MRscore-8CpGs, revised version of continuous mortality risk score with 8 CpGs; FI, frailty index; CVD, cardiovascular disease.
Model 1, adjusted for age, sex, leukocyte composition (not for FI), and batch (not for FI).
Model 2, similar as model 1, additionally adjusted for smoking status (never smoker, former smoker, current smoker), and alcohol consumption (grams per day).