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. 2021 Nov 19;15(1):101270. doi: 10.1016/j.tranon.2021.101270

Fig. 2.

Fig. 2

miR-376a directly regulated the expression of SIRT1 in glioma cells. (A) Schematic showing the binding sequence between miR-376a and the 3′-UTR of SIRT1. (B) The glioma cell lines A172 and LN229 were transfected with a SIRT1-WT or SIRT1-MUT dual-luciferase reporter system and subjected to a dual-luciferase reporter assay. (C) The mRNA level of SIRT1 was detected via qRT–PCR in the glioma cell lines A172 and LN229 after transfection with miR-376a mimic or inhibitor. (D) The protein level of SIRT1 was detected via WB in the glioma cell lines A172 and LN229 transfected with miR-376a mimic or inhibitor. *P< 0.05, **P< 0.01, and ***P< 0.001.