Figure 5.

Tubule-specific LincRNA-p21 deletion reduces apoptosis and ER stress in HFD-fed mice

Representative micrographs of TUNEL assay (A) and quantification of apoptotic cells (arrows) in kidney (B). mRNA levels of apoptotic markers Chop (C), Bax (D), Bcl2 (E), Bax/Bcl2 ratio (F), and ER stress marker Bip (G) in kidneys from HFD-fed mice. Data are expressed as mean ± SEM from each group (n ≥ 5), ∗p < 0.05. Transcripts of LincRNA-p21 (H), IL-6 (I), BIP (J), and CHOP (K) in HK-2 cells pretreated with LNA-lincRNA-p21 GapmeR and stimulated by PA (500 μM) or BSA (0.475%) for 24 h. Results are expressed as mean ± SEM. Experiments were performed in triplicate. ∗p < 0.05 versus Nil, ^#p < 0.05 versus LNA-CTL.