Figure 6.

Activation of p53/LincRNA-p21 axis is regulated through PI3K/AKT/m-TOR/MDM2 signaling cascade in vitro and in vivo

(A) Representative western blots showing phosphorylation levels of PI3K, AKT, mTOR, MDM2, and p53 in kidney tissues. Quantification of the expression of p-PI3K (B), p-AKT (C), p-mTOR (D), MDM2 (E), and p53 (F) by western blots, normalized to β-ACTIN. Data are expressed as mean ± SEM from each group (n ≥ 5), ∗p < 0.01. HK-2 cells pretreated with the p53 inhibitor PFT-α (5 μM) or vehicle were exposed to BSA (0.475%), PA (500 μM), or the p53 activators (RITA at 10 μM and Nutlin-3 at 20 μM) for 24 h, and p53 activity was measured by ELISA (G), and transcripts of LincRNA-p21 (H), BAX (I), and BCL2 (J) were examined by qRT-PCR. Results are expressed as mean ± SEM. Experiments were performed in triplicate. ∗p < 0.05 versus Nil, ^#p < 0.05 versus LNA-CTL.