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. 2000 Aug;20(16):6170–6183. doi: 10.1128/mcb.20.16.6170-6183.2000

FIG. 9.

FIG. 9

HES-1 represses p21 transcription. (A) The endogenous activity of the p21 promoter in PC12 cells (upper half of panel A) was modestly increased by NGF treatment (dark shading), consistent with the induction of p21 by NGF in PC12 cells. Both the endogenous and NGF-induced promoter activities were strongly repressed by HES-1 to below basal levels. Activation of the E-box-enhanced p21 promoter by coexpression of MASH-1 and E47 (lower half of panel A, bracketed) was also further increased by NGF treatment (dark shading). Again, both the MASH-1–E47 and the MASH-1–E47–NGF-activated promoters were strongly repressed to below basal levels by the expression of HES-1. (B) MASH-1–E47-enhanced activation of the p21 promoter (lower section of panel B, bracketed) was repressed by HES-1 in an H-3/4 domain-dependent manner. WT HES-1 strongly, and ΔS HES-1 modestly, repressed the activated p21 promoter, whereas Δ 3/4 HES-1 modestly activated the promoter. ΔR HES-1 conferred no repression activity, and B∗ΔS HES-1 was a modest activator.