Figure 2.

QuPath analysis polygon tool. (a) Left: QuPath annotation toolbox with polygon tool selected. Right: Representative images show how QuPath's polygon tool was used to select either vascular smooth muscle or extravillous trophoblast as the region of interest for analysis. (b) Left: A histogram indicates the staining intensities for all cells within annotated areas for one specimen. Right: Representative images show cell staining intensity for the indicated G-protein coupled receptor 18 stained placental tissues. Cell staining intensity is represented as follows: negative (blue), low (yellow), medium (orange), and high (red)