Fig. 4.

Donor DNA contamination leads to false-positive results. (A) DNA samples from PNA1401 NP–treated cells were digested by nuclease P1. ddPCR failed to detect wild-type sequences from PNA1401 NP–treated samples, in contrast to the spiked-in wild-type genomic DNA control. (B) Serial amounts of donor DNA were spiked into untreated Townes HBB^S/S genomic DNA at 5 ng/μL. After spike-in, 8 μL DNA was used for ddPCR. Error bar indicates SD; n = 3. (C) Proposed model for donor DNA–originated false editing.