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. 2021 Nov 3;118(45):e2103633118. doi: 10.1073/pnas.2103633118

Fig. 3.

Fig. 3.

PIF7 interacts with UBP12 and UBP13 in vitro and in vivo. (A) Interaction between PIF7 and UBP12 or UBP13 in yeast two-hybrid assays. (B) In vitro pull-down assays of UBP12 and UBP13 with PIF transcription factors. * indicates the main band of each MBP-PIF protein. (C) BIFC assay showing PIF7 interacts with UBP12 and UBP13 in Nicotiana benthamiana. (D) co-IP assay of extracts derived from WT (Col-0) and 35S::PIF7-FLASH plants expressing PIF7 with a FLASH tag (3×FLAG, 6×MYC, and 6×HIS). An anti-FLAG M2 affinity agarose gel was used for immunoprecipitation. The input and the immunoprecipitation products were detected by an anti-MYC and an anti-UBP12 antibody, respectively. P-PIF7, phosphorylated form of PIF7.